A tad whiffy due to using beta-mercaptoethanol – hope my lab colleagues don’t mind too much.
Follow the Qiagen protocol pretty much as is, except for the bit where you add ethanol to the QIAshredder flow through.
Instead of adding 96% ethanol, we routinely add 70% ethanol – see point 4 in the picture below. This seems to result in a better yield and quality of RNA. Our samples are for Arabidopsis plants (quite old….around 5 weeks old) and sometimes these plants have experienced constant light so they have lots of polyphenols.
Starting on making GATEWAY gene constructs for making RNA binding protein-GFP fusions for iCLIP and RIP, along the lines of work by the Staiger group (with nice link to ‘Living in an RNA World‘ blog post). Been trying out SnapGene to manipulate and visualise DNA sequences….so far so good (way better than Vector NTI).
Hi – here’s a fun lab-based representation of the circadian clock! It’s been put together using everyday bits-and-pieces found in the lab, and includes things that oscillate, or cycle, and there’s also a balance to represent clock compensation. There’s also some quite random things to add to the concept of the clock responding to chaos. The sculpture has been developed as part of Ally Wallace’s Art-Science collaboration with the Nimmo lab, funded by the Leverhulme Trust. Check out the link, here, for more details.
You can follow abouttimeresearch on Twitter @SpliceTime