RNA day


….a day for making lots of RNA. Using Qiagen’s Plant RNA kit with their lilac and pink tops.

A tad whiffy due to using beta-mercaptoethanol – hope my lab colleagues don’t mind too much.

Follow the Qiagen protocol pretty much as is, except for the bit where you add ethanol to the QIAshredder flow through.

Instead of adding 96% ethanol, we routinely add 70% ethanol – see point 4 in the picture below. This seems to result in a better yield and quality of RNA. Our samples are for Arabidopsis plants (quite old….around 5 weeks old) and sometimes these plants have experienced constant light so they have lots of polyphenols.




Beer o’clock

After a long and difficult day at work, some of us like to treat ourselves to a nice beer. “The clock” by Eden Mill brewery has taken the ‘beer o’clock’ expression almost word for word.

We are now wondering, is this alcohol-seeking behaviour at specific times of the day controlled by our circadian clock?


Photo by Prof John Brown.

Note: we must also remind you that chronic drinking can disrupt circadian rhythms.

Tubus Tubulus


tubes species cropped
Figure(d) Tubus sp. from left to right; Tubus grandis, T. eppendorfis, T. minieppendorfis, T. pcr spp. flatlid and T. pcr spp. roundlid. Botton right; aggregates of T. pcr


Just a bit of fun on this remarkable day – 1st April 2015 – the first description of some new lab species that show remarkable developmental plasticity…..


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