Time to check on how the seeds are doing after the seed spotting.
As you can see some are germinating….but not all.
Is there going to be enough plants for my experiment? I spotted around 250 seeds – it would be great if around half germinated.
After overfilling the tubes with the agar/growth media a cross is sliced into the agar with a sterile blade (see clip above). Doing this his will hopefully help the roots find a way down through the agar/growth media.
Then the Arabidopsis seeds (sterilised in bleach and suspended in sterile water) are pipetted onto the surface of the agar one-by-one (ok, maybe sometimes two-by two) – see clip below.
The trays are then sealed with micropore tape and that’s it – now to wait to see if the seeds germinate – should take around a week to see the emergence of the very young shoots.
Setting up the next experiment. It involves pipetting agar growth media into (literally) 100’s of tubes – see movie clip above. I try to overfill the tubes with the molten agar mixture (this is important for a later step….where I place single Arabidopsis seeds on the agar surface…watch this space).
Science is 99% perspiration, and 1% inspiration….and this is the ‘perspiration’ bit.