….a day for making lots of RNA. Using Qiagen’s Plant RNA kit with their lilac and pink tops.
A tad whiffy due to using beta-mercaptoethanol – hope my lab colleagues don’t mind too much.
Follow the Qiagen protocol pretty much as is, except for the bit where you add ethanol to the QIAshredder flow through.
Instead of adding 96% ethanol, we routinely add 70% ethanol – see point 4 in the picture below. This seems to result in a better yield and quality of RNA. Our samples are for Arabidopsis plants (quite old….around 5 weeks old) and sometimes these plants have experienced constant light so they have lots of polyphenols.